This is a common request for labs using QIAGEN kits (e.g., , QIAprep , QIAquick , RNeasy , DNeasy ). While QIAGEN does not publish exact proprietary formulations for commercial reasons, the general chemical composition of their most common buffers is well-known from patents, safety data sheets (SDS), and lab reverse engineering.
Below is a for preparing equivalent buffers. Always use nuclease-free water for RNA work. 1. Buffer P1 (Resuspension Buffer) Used in: Plasmid Prep (Miniprep, Midi, Maxi) Function: Resuspends cell pellet with RNase A and pH indicator (red). qiagen buffer recipes